Daolio C., Ferreira A. G., Ferreira M. M. C., "1H HR-MAS NMR and Chemometric Analysis to Study Commercial Phytoterapic Plants". Copenhagen, Denmark, 13-15/09/2004: 7th International Conference on Applications of Magnetic Resonance in Food Science: The multivariate challenge. Book of Abstracts, (2004). Poster 10.
10 - 1H HR-MAS NMR AND CHEMOMETRIC ANALYSIS TO STUDY COMMERCIAL PHYTOTERAPIC PLANTS
Cristina Daolio
1, Antonio Gilberto Ferreira1, Marcia M.C.
Ferreira2,
1DQ/UFSCAR-São
Paulo-Brazil, 2IQ/UNICAMP-São Paulo-Brazil
Catuaba is a commercial phytoterapic product and has a
long history of using in Brazilian popular medicine as physical and mental
tonic and especially as a sexual stimulant. However, there is a big confusion
regarding to the correct species sold commercially as Catuaba. One species
is Anemopaegma arvense (Bignoniaceae) called the “official Catuaba”
wich principal active compounds are the alkaloids. Nevertheless, recent
studies showed that in the herbal commerce today the species Trichilia
catigua (Meliaceae) is the most consumed species and the limonoids are
the chemical markers for this family.
In this work we analyse samples with official botanical
classification of both species and eight commercial samples by using 1H
HR/MAS and liquid 1H NMR. The spectra data
showed that after PCA analysis the samples sold in commercial market are
not from Anemopaegma arvense like we will spect. We can also saw that most
of them are very close with Trichilia catigua and few one are completely
different from this two species. In this case, probably it will be a mixture
of differents plants species. The spectral analysis indicated that both
methods, líquid and HR/MAS, lead to the same results. In 1H
HR/MAS NMR technique we used the crude bark tree without any pretreatment
and for liquid experiments a crude methanol extract.
The 1H NMR data were
acquire in BRUKER 9.4 Tesla equipment and all measurements were done in
triplicate using a 4 mm rotor and 5 mm inverse detection probe, respectively
and we use the PCA for chemometric analysis.