Barison A., Alcantara G. B., Tavares L. A., Ferreira A. G., Ferreira M. M. C., de Toledo J. F. F., "DEVELOPMENT OF A METHODOLOGY BY NMR AND CHEMOMETRIC ANALYSIS FOR SOYBEAN QUALITY CONTROL". São Carlos, SP, Brazil, 01-04/12/2004: V Brazilian Meeting on Chemistry of Food and Beverages (V BMCFB), Book of Abstracts (2004). Oral. Session: Quality control.
DEVELOPMENT
OF A METHODOLOGY BY NMR AND CHEMOMETRIC
ANALYSIS
FOR SOYBEAN QUALITY CONTROL
A. Barison1*, G. B. Alcantara1,
L. A. Tavares1, A. G. Ferreira1,
Márcia M. C. Ferreira1
e
J. F. F. de Toledo3
1NMR
Laboratory, Chemistry Department - São Carlos Federal University,
São
Carlos/SP,
Brazil
2Chemistry
Institute - Campinas State University, Campinas/SP, Brazil
3Embrapa
Soja - Empresa Brasileira de Pesquisa Agropecuária, Londrina/PR
ander@dq.ufscar.br
International
foodcrops commodities, mainly European Community,
are more
and more intersted in keep the quality
of the foodcrops. Moreover, consumers are
showing an increasing interest to know the origin
of the products that they buy, this
information is regarded as warranty of quality. This
is responsable for the development
of new analytical methodologies
to verify foodcrops origin as well as its
varieties.
Brazil is the biggest
soybean exporting, so it is so
important the use of these
methodologies for quality control according to
international exigency. In this context,
Nuclear Magnetic Resonance (NMR) together
with Chemometric Analysis became a
very attractive tool. NMR is a powerful task
that permits to obtain all information in a
spectra and HR-MAS technique
provide the possibility of acquiring a
fast NMR
treatment. On the other
hand, Chemometric Analysis, provide a way
to observe
differences in the spectra of very similar samples irrespective
of the large number of
information in the spectra. NMR and Chemometric
Analysis has been successful used
to determine the origin of olive
oil, wines and wheat, used to make flour, only
by
taking NMR spectra acquired directly on wheat flour.
In this work we show a soybean
discrimination by its cultivar
as well as the recognition of
genetically modified
specimen by 1H
HR-MAS NMR spectra acquired directly on the
soybean and by
applying the Chemometric Analysis. For this purpose,
traditional and their respectively
GM soybean samples of three cultivars,
with some environmental conditions, were
analysed. A powder was prepared and
then the NMR spectra were recorded on a
Bruker spectrometer operating at 400.13 MHz for hydrogen
frequently, equipped with
a HR-MAS probe head. Chemometric Analysis
of the spectra allowed to distinguish
samples according to their cultivar
as well as GM or traditional. This methodology
may help Brazil to certificate its agricultural products,
not only soybean, once a single
HR-MAS NMR spectra is necessary.