Marcucci M. C., Ferreres F., Ferreira M. M. C., Custódio A. R., Bankova V. S., Garcia-Viguera C., “Evaluation of phenolic compounds in Brazilian propolis from different geographic regions, using principal components analysis”. Vancouver, Canada, 12-17/09/1999: XXXVI International Apicultural Congress (Apimondia '99), Book of Abstracts, 295 (1999). Poster 159.

M.C. Marcucci*, F. Ferreres*, M.M.C. Ferreira**, R. Custódio***, V.S. Bankova**** and C.
Garcia-Viguera*; *Centrol de Edafologia y Biologia Aplicada del Seguar, Murcia, Spain, ** Instituto de
Química - UNICAMP, Campinas SP, Brazil, ***Universidade São Francisco, Bragança Paulista, SP,
Brazil. ****Institute of Organic Chemistry with Centre of Phytochemistry, Sofia, Bulgaria.

Forty propolis samples,   originating from different  locations  in  South  and  South  Eeast  Brazil,  were anal-
lyzed  by  HPLC  and  18  compounds of interest studied, including:  caffeic,  pherulic  and  p-coumaric  acid,
3-prenyl-4-hydroxycinnamic     acid     (PHCA),       3,5-diprenyl-4-hydroxy-cimiamic       acid        (DHCA),
2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyrane     (DCBEN),     2,2-dimethyl-6-carboxy-ethenyl-8-prenyl-
2H-1-benzopyrane (DCPBEN),  pino-banxin, a caempherol derivative  KV and compound  E  (still unindenti-
fied).   Principal component analysis  (PCA)  indicated 3 types of propolis.   Samples of  SE  group are rich in
KV. Samples from SE group have a high content of DCPBEN but low concentration of KD and DCBEN. SII
group displays a high concentration of DCBEN,  DHCA,  p-coumaric acid and DCPBEN.   The first two PCs
seem to  have some  relationship to  the plant origin of  propolis.   Hierarchical  Cluster  Analysis  showed  the
same trend of the PCA. These two results are of great importance with respect to the identification of propolis
plant sources and its proper standardization.
 
 
 
 
 
 

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