Barison A., Alcantara G. B., Ferreira A. G., de Toledo J. F. F., Ferreira M. M. C., "GM Soybean Crops Recognized by ¹H HR-MAS NMR and Chemometric Analysis". Copenhagen, Denmark, 13-15/09/2004: 7th International Conference on Applications of Magnetic Resonance in Food Science: The multivariate challenge. Book of Abstracts, (2004). Poster 13.

13 - GM SOYBEAN CROPS RECOGNIZED BY ¹H HR-MAS NMR AND CHEMOMETRIC ANALYSIS
 

Andersson Barison¹, Glaucia Braz Alcantara², Antonio Gilberto Ferreira², José Franciso Feraz de Toledo³, Marcia Miguel
Castro Ferreira⁴
¹Universidade Estadual de Mato Grosso do Sul – UEMS, ²Universidade Federal de São Carlos – UFSCar, ³Empresa
Brasileira de Pesquisa Agropecuária – Embrapa, ⁴Universidade Estadual de Campinas - UNICAMP

In the last few years genetically modified (GM) crops, mainly herbicide tolerant soybean, had a rapidly acceptance by producers in several places in the world. Because genetic modification in food crops is a relatively new technology we must to concern about its environmental and human beings impact. Moreover, the use of GM crops in many countries, mainly in European Union, must follow regulations and it is very important to have tools for this.
In this work we show a new methodology to recognize GM soybean crops by ¹H HR-MAS NMR and chemometric analysis. For this propose we use two samples of GM and one of “conventional” soybean varieties with identical origin and cultivated at the same conditions. The ¹H HR-MAS NMR spectra, and the PCA allowed to distinguish the GM samples from their traditional specimens with a good results. This data also were used to built a classify model which was able to classify some unknown samples. This results show us that NMR could be a very attractive analytical technique because its permits to obtain a fast spectra with resolution enough to distinguish samples with small differences without sample pretreatment. Moreover, ¹H NMR interpretation of observed differences in the spectra of GM and no GM crops could give important informations about the compounds involved in this discrimination.
The ¹H NMR data were acquired in BRUKER 9.4 Tesla equipment and all measurements were done in triplicate using a 4 mm rotor at 298K and we use the PCA for chemometric analysis.