M. V. Guterres, P. O. L. Volpe, M. M. C. Ferreira, "Analysis of Pigments Produced by Chromobacterium Violaceum in Aerobic Fermentor With PARAFAC Model and 2D Fluorescence". Lisbon, Portugal, 20-23/09/2004: Ninth International Conference on Chemometrics in Analytical Chemistry (CAC IX, CAC-2002). Program and Abstracts Book, (2004). Poster 183. Section: Poster Session 5 - Chemometrics & Process / Industrial Applications.

ABSTRACT

Analysis of Pigments Produced by Chromobaterium violaceum in Aerobic Fermentor with PARAFAC Model and 2D Fluorescence. Guterres, M.V.¹; Volpe, P.O.L.²; Ferreira M.M.C.³¹Instituto de Química - UNICAMP- Campinas, Brasil. guterres@iqm.unicamp.br. ²Instituto de Química - UNICAMP- Campinas, Brasil. ³Instituto de Química - UNICAMP- Campinas, Brasil. Keywords: C. violaceum, PARAFAC, 2D fluorescence.  Chromobacterium violaceum is an important microorganism for biotechnology research. In aerobic fermentation, this microorganism produces pigments and other substances with bactericidal, trypanocidal and antiviral activities. In this work, the fermentations were performed in a bioreactor and monitored by 2D fluorescence to follow the consumption of amino acids and the production of pigments during the fermentation. Samples were extracted in regular intervals of time during 24 or 42 hours. Fluorescence spectra were obtained in the intervals of 240 – 390 nm for excitation and 290-540 nm for emission. Constrained PARAFAC model was fitted to the Three Way data set (samples × excitation × emission). The results obtained, show that two pigments, violacein and deoxiviolacein, are produced in the first 42 hours of fermentation. Other substances such as amino acids, NADH and ATP were also identified. The microorganism quickly assimilates the amino acids in the first 24 hours of fermentation. On the other hand, the production of pigments is faster after 15 hours of fermentation, when most of the amino acids are consumed. This observation is in agreement with other experimental evidences that C. violaceum uses tryptophane for the production of violacein. The proposed methodology was effective in the exploratory analysis of metabolism of C. violaceum and can be extended to other microorganisms. Acknowledgements: FAPESP

Keywords: c. violaceum, parafac, 2d fluorescence

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Corresponding author: MÁRCIA M. C. FERREIRA (marcia@iqm.unicamp.br)